 |
| |
The Mass Spectroscopy Core Facility
provides the center with the capability to quantify the stable isotope
metabolites in Project 1 as well as to develop new methodology to
quantify protein synthesis using mass spectroscopy and in particular,
the newly acquired Q-TOF methods. Similarly, in Project 3, the Q-TOF
instrumentation facilitates the identification of phosphorylation
sites of IRS-1 proposed in the project. The facility is also shared
for the research in Projects 2 and 4. The NMR Spectroscopy Core Facility
helps us develop methods to examine changes in metabolism and biomarkers
of tissue response to burn injury in human and model systems.
Knowledge acquired from the NMR studies of human fluids, a technique
highly suitable for routine clinical application, will be applied
toward the understanding of the role of the liver in glucose metabolism
in response to burn injury.  Also,
specific NMR methodologies will be developed for imaging apoptosis
and monitoring mitochondrial function in Project 4. Application
of this new methodology in Projects 1 and 4 is important because
it replaces our need to separate the tissues and fluids into cationic,
anionic, and neutral species; this latter methodology has proven
to be highly problematic and a major obstacle to progress in this
area of research.
Furthermore, as methodology improves, it should be possible to
make many of these measurements in vivo, non-invasively and these
measures can be compared with similar measurements using PET technologies.
|
